1. Transverse line of stout, socketed setae on occiput partial or complete, extending to area of ecdysial suture (Fig. 2.1.1; Fig. 2.1.2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Transverse line of stout, socketed setae on occiput absent or confined to just medial of compound eye (Fig. 2.1.3, Fig. 2.1.4) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2. Two ocelli (Fig 2.1.5) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Neoperla
Three ocelli, some with small, obscure median ocellus (Fig 2.1.6) . . . . . . . . . . . . . . . 3
3. Anal gills present (Fig. 2.1.7). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Anal gill absent (Fig. 2.1.8). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
4. Row of socketed setae on occiput complete and tight row to ecdysal surture (Fig.2.1.9); abdomen and thorax not covered with heavy socketed setae (i.e., not freckled)(Fig.2.1.10) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Agnetina
Row of socketed setae on occiput incomplete, open in places, but reaching or nearly so, ecdysal suture (Fig. 2.1.11); abdomen and sometimes thorax covered with heavy socketed setae, giving the impression of a freckled surface (Fig. 2.1.12) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Perlesta Group A
5. Transverse line of stout, socketed setae on occiput somewhat wavy and broken at midline; body uniformly light brown; head unicolorous; possibly extirpated from Ohio . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Attaneuria ruralis
Transverse line of stout, socketed setae on occiput not wavy and not broken at midline (Fig. 2.1.13); body either uniformly dark or patterned (Fig. 2.1.14); head often with 5 star-like light markings around median ocellus (Fig. 2.1.15) . . . . . . . . . . Paragnetina
6. Marginal setae of lacinia stout and few, forming a distinct cluster below second tooth (Fig. 2.1.16) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Perlesta Group B
Marginal setae of lacinia many, may be stout, forming a near continuous line to the base of lacinia (Fig. 2.1.17) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
7. Femora and tibia with dorsal and ventral fringes of long, silky setae (Fig. 2.1.18); habitus long and narrow to live between gravel spaces (Fig. 2.1.19) . . . . . . Perlinella
Femora and tibia with only dorsal fringes of long, silky setae (Fig. 2.1.20); habitus stout (Fig. 2.1.21) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
8. Dorsum of head with large area of yellow pigment anterior to median ocellus
(Fig 2.1.22) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Eccoptura xanthenes
Dorsum of head mostly brown, frequently with a variable yellow M-shaped mark anterior to median ocellus (Fig 2.1.23, Fig 2.1.24) . . . . . . . . . . . . . . . . . Acroneuria
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Key to the Genera of Ohio Perlidae Nymphs
(Modified from Stewart and Stark (2002), Hitchcock, 1974, and Poulton and Stewart, 1991)